|
| Status |
Public on May 01, 2013 |
| Title |
42C control batchB rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Ba/F3
|
| Organism |
Mus musculus |
| Characteristics |
treatment: heat shocked for 15 minutes at 42C
|
| Treatment protocol |
Cells were either untreated (negative), heat shocked for 15 minutes at 42C, 47C or 52C or actuated continuously on device for 15 minutes at 400Vpp and either 1 kHz or 18 kHz sinusoidal frequencies
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed and total RNA extracted and purified using Arcturus Picopure RNA Isolation kits (Applied Biosystems) according to manufacturer protocols. Quality control by Agilent Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
RNA samples were labelled using Illumina TotalPrep-96 RNA Amplification Kit Lot #1108030 Ambion as per protocol. 1.5 ng cRNA randomized and hybridized
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scan protocol
|
| Description |
6903016035_B
Batch B Replicate 2
|
| Data processing |
Data analysis was conducted using Genespring v.11.5.1. Two batches of microarray data were normalized using the Empirical Bayes ComBat accommodation of batch effects using an R script. Each array batch contained 3 untreated control biological replicates for cross-batch normalization. Normalization by standard quantile method followed by a “per probe” median centered normalization
|
| |
|
| Submission date |
Jan 15, 2013 |
| Last update date |
May 01, 2013 |
| Contact name |
Sam Au |
| E-mail(s) |
sam.au@utoronto.ca
|
| Organization name |
University of Toronto
|
| Street address |
160 College St. Room 440
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5S 3E1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE43507 |
Genome-level Cell Responses to Digital Microfluidic Manipulation |
|
