|
| Status |
Public on Jan 17, 2013 |
| Title |
Daphnia magna MTBE rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Daphnia magna exposed for 24h
|
| Organism |
Daphnia magna |
| Characteristics |
chemical: MTBE
age: 14 days
exposure time: 24h
dose: 145 mg/L
|
| Treatment protocol |
Twenty daphnids (Two-week old) were exposed to 1 L of COMBO medium containing 1/10 LC50 concentration of each chemical with four replicates
|
| Growth protocol |
D. Magna were obtained from Aquatic Research Organisms (Hampton, NH) and cultured at 20C in modified COMBO medium. Culturing medium was renewed three times a week, and Pseudokirchneriella subcapitata (formerly Selenastrum capricornutu) and YCT added for food.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 24 h exposure, total RNA was extracted with Trizol (Invitrogen, CA, USA) according to the standard method.
|
| Label |
Cy3
|
| Label protocol |
Agilent 1 colour Quick-Amp Labelling Protocol
|
| |
|
| Hybridization protocol |
Agilent 1 colour Quick-Amp Labelling Protocol
|
| Scan protocol |
The scanning and feature extraction were performed using GenePix 4000B (Axon, USA) and GenePix Pro 6.0 software (Axon, USA).
|
| Description |
Control-Media
|
| Data processing |
Raw microarray data was read and normalized against their respective controls (listed in the description field) using loess within the statistical environment R using the marray and limma packages.
|
| |
|
| Submission date |
Jan 16, 2013 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Philipp Antczak |
| E-mail(s) |
p.antczak@liv.ac.uk
|
| Organization name |
University of Liverpool
|
| Street address |
Crown Street
|
| City |
Liverpool |
| State/province |
Merseyside |
| ZIP/Postal code |
L69 7ZB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16518 |
| Series (1) |
| GSE43564 |
The Molecular Toxicity Identification Evaluation (mTIE) Approach Predicts Chemical Exposure in Daphnia magna |
|
