|
Status |
Public on Jun 30, 2013 |
Title |
WT warm, biological rep2 |
Sample type |
RNA |
|
|
Source name |
Arabidopsis thaliana Col-0 WT grown at 22°C
|
Organism |
Arabidopsis thaliana |
Characteristics |
tissue: entire aerial part cultivar/ecotype: Col-0 genotype/variation: wild type
|
Treatment protocol |
Plants were treated at 4°C under a 12 hr photoperiod for 24 h
|
Growth protocol |
Plants were grown in soil at 22°C under a 12 hr photoperiod for 3 weeks
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the RNeasy plant mini kit (Qiagen)
|
Label |
biotin
|
Label protocol |
RNA was amplified and biotinylated using a MessageAmp kit (Ambion) according to the manufacturer's protocol
|
|
|
Hybridization protocol |
Hybridization and washing were carried out according to the Gene Chip manufacturer's protocol (Affymetrix)
|
Scan protocol |
Scan protocols were carried out according to the Gene Chip manufacturer's protocol (Affymetrix)
|
Description |
Gene expression data from Arabidopsis thaliana Col-0 WT at 22°C
|
Data processing |
The data were analyzed with LIMMA package of Bioconductor using GCRMA normalization procedure.
|
|
|
Submission date |
Jan 28, 2013 |
Last update date |
Aug 15, 2018 |
Contact name |
Sunchung Park |
E-mail(s) |
Sunchung.park@usda.gov
|
Organization name |
USDA -ARS
|
Department |
ARS-NEA
|
Lab |
SPCL
|
Street address |
10300 Baltimore ave, Bldg001
|
City |
Beltsville |
State/province |
MD |
ZIP/Postal code |
20705 |
Country |
USA |
|
|
Platform ID |
GPL198 |
Series (1) |
GSE43818 |
Expression data from WT, camta1/2, camta1/3, camta2/3, camta1/2/3 mutants |
|
Relations |
Reanalyzed by |
GSE118579 |