|
| Status |
Public on Jun 30, 2013 |
| Title |
camta1/2/3 24h cold biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis thaliana camta1/2/3 mutant grown at 22°C and treated at 4°C for 24 hrs
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: entire aerial part
cultivar/ecotype: Col-0
genotype/variation: camta1/2/3 mutant
|
| Treatment protocol |
Plants were treated at 4°C under a 12 hr photoperiod for 24 h
|
| Growth protocol |
Plants were grown in soil at 22°C under a 12 hr photoperiod for 3 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy plant mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and biotinylated using a MessageAmp kit (Ambion) according to the manufacturer's protocol
|
| |
|
| Hybridization protocol |
Hybridization and washing were carried out according to the Gene Chip manufacturer's protocol (Affymetrix)
|
| Scan protocol |
Scan protocols were carried out according to the Gene Chip manufacturer's protocol (Affymetrix)
|
| Description |
Gene expression data from Arabidopsis thaliana camta1/2/3 mutant at 4°C for 24 hrs
|
| Data processing |
The data were analyzed with LIMMA package of Bioconductor using GCRMA normalization procedure.
|
| |
|
| Submission date |
Jan 28, 2013 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Sunchung Park |
| E-mail(s) |
Sunchung.park@usda.gov
|
| Organization name |
USDA -ARS
|
| Department |
ARS-NEA
|
| Lab |
SPCL
|
| Street address |
10300 Baltimore ave, Bldg001
|
| City |
Beltsville |
| State/province |
MD |
| ZIP/Postal code |
20705 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE43818 |
Expression data from WT, camta1/2, camta1/3, camta2/3, camta1/2/3 mutants |
|
| Relations |
| Reanalyzed by |
GSE118579 |
