|
| Status |
Public on Jan 31, 2013 |
| Title |
iPS-NHDF-1 [SNP] |
| Sample type |
genomic |
| |
|
| Source name |
adult normal human dermal fibroblast-derived induced pluripotent stem cell line
|
| Organism |
Homo sapiens |
| Characteristics |
culture medium: mTeSR-1 + matrigel
|
| Treatment protocol |
untreated cell samples; Fibroblasts grown in ADMEM + 10% FCS; iPSc lines all grown in mTeSR-1 medium + ES-qualified matrigel prior to harvest
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted using QIAGEN DNeasy blood and tissue kit
|
| Label |
biotin (C+G) and dinitrophenyl (A+T)
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridisation protocol
|
| Scan protocol |
Standard Illumina scanning protocol for BeadChip Arrays
|
| Description |
SAMPLE 2
|
| Data processing |
The scanned data files were processed by GenomeStudio Genotyping module (v1.9), using the cluster file HumanCytoSNP-12v2-1_H.egt provided by Illumina. Raw data was also uploaded to KaryoStudio (Illumina) for generation of karyotype reports (see publication).
|
| |
|
| Submission date |
Jan 30, 2013 |
| Last update date |
Jan 31, 2013 |
| Contact name |
Sally A Cowley |
| E-mail(s) |
sally.cowley@path.ox.ac.uk
|
| Phone |
+44 (0) 1865 275600
|
| Organization name |
University of Oxford
|
| Department |
Sir William Dunn School of Pathology
|
| Lab |
James Martin Stem Cell Facility
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13829 |
| Series (2) |
| GSE43902 |
SNP data from human fibroblasts and induced Pluripotent Stem cells |
| GSE43904 |
SNP and expression data from human induced Pluripotent Stem cells derived from normal human dermal fibroblasts |
|
