|
| Status |
Public on Jul 27, 2006 |
| Title |
LPS + CyP 3hr 1 |
| Sample type |
RNA |
| |
|
| Source name |
Monocyte derived dendritic cells
|
| Organism |
Homo sapiens |
| Characteristics |
Monocyte derived dendritic cells were generated by culturing human monocytes isolated from buffy coats using CD14 microbeads (Miltenyi)
|
| Treatment protocol |
cells were plated in fresh medium and incubated for 4h at 37°C before stimulation with 0.5 µg/ml S. abortus equi S-form LPS, pure TLR4 agonist (Alexis), together with 20 µg/ml CyP (Cyanobacterial Product) for 3h
|
| Growth protocol |
CD14+ cells from PBMC were cultured for five days in 6-well plates in RPMI, 10% fetal bovine serum (Hyclone) supplemented with GM-CSF 50ng/ml and IL-4 1,000 U/ml. On day 5 monocyte-derived dendritic cells were harvested and replated at 0.5 106 cells/ml (1.5 106 cells/well).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cells were harvested and lysed using TRIzol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip Expression 3’ Amplification One-Cycle Target Labeling kit from about 5ug of total RNA
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures, washes performed using Fluidics Station 400
|
| Scan protocol |
standard Affymetrix procedures using a GeneChip Scanner 3000
|
| Description |
This sample is also known as IOSI 05-052
|
| Data processing |
Affymetrix GCOS 1.2
|
| |
|
| Submission date |
May 01, 2006 |
| Last update date |
Jul 27, 2006 |
| Contact name |
Francesco Bertoni |
| E-mail(s) |
francesco.bertoni@ior.usi.ch
|
| Phone |
+41 91 820 0367
|
| Organization name |
IOR
|
| Department |
Lymphoma & Genomics Research Program
|
| Street address |
Via V. Vela 6
|
| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE4748 |
A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation |
|
