|
Status |
Public on Jun 01, 2017 |
Title |
Scn5adelta/+ mouse 2 left ventricle |
Sample type |
RNA |
|
|
Source name |
Mouse left ventricle
|
Organism |
Mus musculus |
Characteristics |
genotype: Scn5adelta/+ KI/heterozygote/150-5KPQ-1507 del genetic background: Swiss Agouti age: 12 months tissue: Left ventricle + Septum
|
Growth protocol |
12 months old mice
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Rneasy fibrous tissue mini ki (Qiagen with DNase I treatment on column) in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
biological replicate 2
|
Data processing |
The data were normalised using cubic spline normalization with Geome Studio Software (Illumina, 2010).Differential gene expression was assessed employing the Illumina custom algorithm
|
|
|
Submission date |
Jan 31, 2013 |
Last update date |
Jun 01, 2017 |
Contact name |
Ilaria Piccini |
E-mail(s) |
ilaria.piccini@ukmuenster.de
|
Organization name |
UKM
|
Street address |
Albert-Schweitzer-Campus 1
|
City |
Muenster |
ZIP/Postal code |
48149 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE43967 |
Selective increase in late sodium current leads to left ventricular fibrosis with ageing and both excessive hypertrophy and fibrosis with pressure overload in a knock-in murine model |
|