ES cells were washed by DPBS (GIBCO), trypsinized, and collected by centrifugation.
Growth protocol
Monkey parthenogenic haploid embryonic stem cells (PG-haESCs) were cultured on feeder in ES medium supplemented with Y27635 and Thiazovivin.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted and purified using TRIZOL Reagent (Cat#15596-018, Life technologies, Carlsbad, CA,US) according to the manufacturer's instructions.
Label
biotin
Label protocol
Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Hybridization protocol
Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079,Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Scan protocol
Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
Description
M5-P12 Gene expression data from ICSI-derived ESCs
Data processing
Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
Submission date
Feb 01, 2013
Last update date
Jun 26, 2013
Contact name
Linyu Shi
Organization name
Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
Lab
Mechanism and Application of Somatic Reprogramming
