|
| Status |
Public on May 12, 2020 |
| Title |
E742N |
| Sample type |
RNA |
| |
|
| Source name |
adjacent normal tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: adjacent normal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Two methods were used to extract total RNA. One is by standard Trizol method and Second is by AllPrep DNA/RNA/Protein Mini Kit from QIAGEN. After extractiong, all are followed with qualification check by Agilent 2100.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix).
|
| |
|
| Hybridization protocol |
For mRNA array, total RNA was reverse transcripted to cDNA, reverse transcripted to labelled aRNA, purified, fragmented, and hybridized to each array chip according to the manufacturer's instructions.
|
| Scan protocol |
The mRNA arrays were washed using Affymetrix fluidics stations, and scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
frozen tissue extracted by Trizol
|
| Data processing |
Microarray data were normalized using the RMA method .
|
| |
|
| Submission date |
Feb 01, 2013 |
| Last update date |
May 12, 2020 |
| Contact name |
Howard Yang |
| E-mail(s) |
yanghow@mail.nih.gov
|
| Phone |
2402765257
|
| Organization name |
NCI
|
| Street address |
9609 Medical Center Dr.
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE44021 |
Intergrated analysis of array-miRNAs and matched Affymetrix gene |
| GSE67269 |
Integrative analysis of miRNAs and matched gene expression in ESCC |
|
