|
| Status |
Public on Feb 03, 2013 |
| Title |
Blastocyst 2 |
| Sample type |
SRA |
| |
|
| Source name |
Ovary
|
| Organism |
Bos taurus |
| Characteristics |
tissue: blastocyst
|
| Growth protocol |
24 hour maturation following follicular aspiration of oocyte; IVF; and then culture in KSOM+BSA for 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Arcturus Picopure (RNA)
Nugen Ovation V1 (cDNA) followed by Nugen Encore (Library)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
Filtering for quality by Galaxy FastQC
RNA-Seq analysis using CLC Genomics Workbench Ver. 4.7
Genome_build: Btau 4.0, Btau4.2 and UMD3.1
Supplementary_files_format_and_content: Ensembl Gene ID and RPKM
|
| |
|
| Submission date |
Feb 01, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
James Lee Chitwood |
| E-mail(s) |
jlchitwood@ucdavis.edu
|
| Organization name |
University of California, Davis
|
| Department |
Animal Science
|
| Street address |
1 Shields Avenue
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95691 |
| Country |
USA |
| |
|
| Platform ID |
GPL15750 |
| Series (1) |
| GSE44023 |
RNA-seq analysis of single bovine blastocysts |
|
| Relations |
| SRA |
SRX222006 |
| BioSample |
SAMN01909098 |
