|
| Status |
Public on Jun 05, 2013 |
| Title |
pre-iPSC- siRNA to luciferase- biological replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
pre-iPSC- siRNA to luciferase- biological replicate2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: pre-iPSCs
sirna mediated knockdown: control
|
| Treatment protocol |
pre-iPSCs were treated with indicated siRNA for 3 days and then RNA was extracted
|
| Growth protocol |
pre-iPSCs we re grown in standard embryonic stem cell media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction was carried out according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridisation was performed using Affymetrix protocol
|
| Scan protocol |
Gene ChiPs were scanned with a Gene Array Scanner
|
| Description |
pre-iPSC-control 2
|
| Data processing |
CEL files generated were normalized by RMA normalization from the R statistical package
|
| |
|
| Submission date |
Feb 05, 2013 |
| Last update date |
Jun 05, 2013 |
| Contact name |
Rupa Sridharan |
| E-mail(s) |
rsridharan2@wisc.edu
|
| Organization name |
UW-Madison
|
| Street address |
2118 Wisconsin Institute for Discovery
|
| City |
Madison |
| ZIP/Postal code |
53715 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE44084 |
Expression data from pre-iPSCs with a control, histone methyltransferase or Cbx3 (HP1g) knockdown |
|
