|
| Status |
Public on Feb 12, 2013 |
| Title |
14 day control wild type rep3 |
| Sample type |
RNA |
| |
|
| Source name |
14 day control wild type rep3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
age: 6 months
tissue: triceps surae muscle
|
| Treatment protocol |
Animals were subjected to unilateral sciatic nerve resection and RNA isolated from the denervated muscles after 3 or 14 days; control animals were housed in parallel without denervation.
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalization with Illumina BeadStudio v3.2.3 software and background was subtracted
|
| |
|
| Submission date |
Feb 08, 2013 |
| Last update date |
Feb 12, 2013 |
| Contact name |
J. David Furlow |
| E-mail(s) |
jdfurlow@ucdavis.edu
|
| Organization name |
UC Davis
|
| Street address |
One Shields Avenue
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL6105 |
| Series (2) |
| GSE44205 |
Genome-wide analysis of denervation induced gene expression in mouse triceps surae muscle |
| GSE44259 |
Genome-wide analysis of denervation and dexamethasone-responsive gene expression in mouse triceps surae muscle |
|
