|
| Status |
Public on Aug 24, 2013 |
| Title |
Knockout liver DEN-injected 12weeks repl1 |
| Sample type |
RNA |
| |
|
| Source name |
Knockout liver DEN-injected 12weeks repl1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
genotype/variation: Hmgn1-/-
treatment: injection of 10 µg/g body weight of N-nitrosodiethylamine
|
| Treatment protocol |
At age of 4 weeks HMGN1 wildtype and knockout males received an injection of 10 µg/g body weight of N-nitrosodiethylamine or saline as a control.
|
| Growth protocol |
Hmgn1+/+ and Hmgn1-/- littermates were generated by crossing heterozygous Hmgn1+/- male and female, DNA-tail-genotyping F1 mice and further propagating Hmgn1+/+ and Hmgn1-/- homozygotes separately
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen liver tissue with TRIzol followed by purification using RNeasy Kit.
|
| Label |
biotin
|
| Label protocol |
GeneChip One-Cycle Target Labeling and Control Reagents (Affymetrix, P/N 900493).
|
| |
|
| Hybridization protocol |
GeneChip Hybridization, Wash and Stain Kit: Affymetrix, P/N 900720, suppliers recommendations, mouse 430.2 Affymetrix expression array
|
| Scan protocol |
Agilent GeneArray Scanner
|
| Data processing |
Mouse Genome 430 2.0 array has 45101 probe sets associated with approximately 20000 Mouse Genome Informatics (MGI) gene identifiers. Probe sets were mapped to MGI identifiers using information provided by the Jackson Laboratory (http://www.informatics.jax.org/). Values in the Sample data tables were derived from MAS5 algorithm.
|
| |
|
| Submission date |
Feb 15, 2013 |
| Last update date |
Aug 24, 2013 |
| Contact name |
Yuri Postnikov |
| E-mail(s) |
PostnikY@mail.nih.gov
|
| Phone |
301-496-2885
|
| Organization name |
NIH
|
| Street address |
37 Convent Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE44356 |
Expression data from wild-type and HMGN1 knockout mice injected with N-nitrosodiethylamine |
|
