|
| Status |
Public on Sep 27, 2013 |
| Title |
ESC shDnmt3L3 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ESC_shDnmt3L3
|
| Organism |
Mus musculus |
| Characteristics |
cell type: E14 embryonic stem cells
transfected with: shDnmt3L
|
| Treatment protocol |
Cells were transfected with shRNAs and selected with Puromicin for 3 days before RNA extraction
|
| Growth protocol |
Embryonic stem cells were cultured in DMEM high glucose medium (Invitrogen) supplemented with 15% FBS (Millipore), 0.1mM nonessential amino acids (Invitrogen), 1mM sodium pyruvate (Invitrogen), 0.1mM 2-mercaptoethanol, 1500U/ml LIF (Millipore), 25 U of penicillin/ml, and 25 µg of streptomycin/ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIzol reagent from Invitrogen.
|
| Label |
Cy3
|
| Label protocol |
RNA library were performed using Illumina Total Prep RNA Amplification Kit following the manufacturing protocol
|
| |
|
| Hybridization protocol |
Direct Hybridization of RNA library was performed using Standard Operating Procedures in Whole-Genome Gene Expression Direct Hybridization Assay Guide (Illumina)
|
| Scan protocol |
Chips were scanned using Illumina HiScan software
|
| Description |
shDnmt3L3-2
|
| Data processing |
Data were normalized using quantile normalization and background subtraction by default parameters in Illumina Genome Studio (V.2011.1) Module Gene Expression (V.1.9.0).
|
| |
|
| Submission date |
Feb 25, 2013 |
| Last update date |
Sep 27, 2013 |
| Contact name |
Francesco Neri |
| E-mail(s) |
francesco.neri@unito.it
|
| Organization name |
University of Torino
|
| Street address |
Via Nizza 52
|
| City |
Torino |
| State/province |
Italy |
| ZIP/Postal code |
10126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE44643 |
Dnmt3L-dependent regulation of DNA methylation promotes stem cells differentiation toward primitive germinal cells [Expression array] |
| GSE44644 |
Dnmt3L-dependent regulation of DNA methylation promotes stem cells differentiation toward primitive germinal cells |
|
