|
Status |
Public on Jun 21, 2013 |
Title |
ChorionicVilli_EOPET_13 (methylation) |
Sample type |
genomic |
|
|
Source name |
placental chorionic villus
|
Organism |
Homo sapiens |
Characteristics |
tissue: placental chorionic villus condition: EOPET gestational age (weeks): 28.56 fetus gender: Male
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was purified using the Dneasy Blood and Tissue kit (Qiagen) followed by bisufite conversion using the EZ DNA methylation kit (Zymo) following manufacturer's protocols.
|
Label |
Cy5 and Cy3
|
Label protocol |
Illumina HumanMethylation450 BeadChip Protocol
|
|
|
Hybridization protocol |
Illumina HumanMethylation450 BeadChip Protocol
|
Scan protocol |
Arrays were imaged using Illumina iScan on a two-color channel
|
Description |
Sample name: PM80
|
Data processing |
GenomeStudio 2011.1 software and R version 2.14.0 Unmethylated and methylated signal intensities matrix linked as supplementary file on the Series record.
|
|
|
Submission date |
Feb 26, 2013 |
Last update date |
May 17, 2016 |
Contact name |
Wendy Robinson |
E-mail(s) |
wprobinsonlab@gmail.com
|
Organization name |
University of British Columbia
|
Department |
Medical Genetics
|
Lab |
Robinosn
|
Street address |
950 W 28th Ave
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V5Z 4H4 |
Country |
Canada |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE44667 |
DNA methylation in Early Onset Preeclamptic placentas |
GSE44712 |
Early-Onset Preeclampsia Placentas and Controls |
|