|
| Status |
Public on Mar 15, 2013 |
| Title |
LH - rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
adult bovine ovarian theca cels in primary culture
|
| Organism |
Bos taurus |
| Characteristics |
protocol: LH
cell type: bovine theca cells
|
| Treatment protocol |
Cells were were plated out in in 24-well plates (0.5 x106 viable cells/ml/well) and cultured for 6 days under defined serum-free conditions with treatments present on days 3-6 inclusive. TC were treated for 4 days with BMP6 (10 ng/ml) under both basal and LH-stimulated conditions (± 160 pg LH/ml).
|
| Growth protocol |
Bovine ovarian theca interna cells (TC) were isolated from adult bovine ovaries obtained from the slaughterhouse
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Ribopure RNA isolation kit (Ambion) according to the manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix workflow by accredited service provider
|
| |
|
| Hybridization protocol |
Standard Affymetrix workflow by accredited service provider
|
| Scan protocol |
Standard Affymetrix workflow by accredited service provider
|
| Data processing |
Raw hybridization data were stored as .cel files and loaded into Genespring GX software for probe summarization (robust multi-array averaging, RMA algorithm), filtering and statistical analysis using the GeneSpring ‘biological significance’ workflow utility for a gene-level experiment.
Affymetrix Expression Console rma.chp files were provided since submitter doesn't have access to Genespring GX software (licence expired since analysis was done).
|
| |
|
| Submission date |
Feb 27, 2013 |
| Last update date |
Mar 15, 2013 |
| Contact name |
Phil G Knight |
| E-mail(s) |
p.g.knight@reading.ac.uk
|
| Organization name |
University of Reading
|
| Street address |
Hopkins Building, Whiteknights
|
| City |
Reading |
| ZIP/Postal code |
RG6 6UB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE44704 |
Expression data from bovine ovarian theca cells in primary culture |
|
