The mouse cortex samples were obtained from P1 C57/B6 mice (n = 19), which were purchased from Charles River Laboratories (Charles River Laboratories, Inc., Willmington, MA).The mouse cortex was used as a reference sample. Universal mRNA reference was still being developed when the study started, so it was not utilized. All samples were placed in TRIzol reagent (Invitrogen, Carlsbad, CA) and stored at -80ºC before RNA extraction. Total RNA was extracted with TRIzol reagent according to the manufacturer's instructions. For a quality control measure of the samples, total RNA was ran through a 1% agarose gel and using the 2100 Agilent BioAnalyzer System (Agilent Technologies, Palo Alto, CA) to check for possible contamination and degradation.
Extracted molecule
total RNA
Label
Biotin
Label protocol
CodeLink Expression Assay protocol
Hybridization protocol
CodeLink Expression Assay protocol
Scan protocol
CodeLink Expression Assay protocol
Description
MC technical replicate 5
Data processing
Signal intensities across microarrays were normalized using the quantile normalization (www.bioconductor.org).
