|
| Status |
Public on May 15, 2014 |
| Title |
Patient_02_miRNA |
| Sample type |
RNA |
| |
|
| Source name |
Liver tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Liver biopsy sample
Sex: M
outcome: NVR
array_lot: 31078
|
| Treatment protocol |
miRNA expression profiles of liver tissues from 12 patients with chronic hepatitis C were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A global miRNA expression analysis was performed using 1 µg of total RNA and the miRCURY LNA microRNA Array series (Exiqon, Vedbaek, Denmark).
|
| Label |
Hy3
|
| Label protocol |
Total RNA was labeled with Hy3 fluorescent label using the miRCURY LNA microRNA Power Labeling kit (Exiqon).
|
| |
|
| Hybridization protocol |
Hybridization to the miRNA microarrays were performed following the procedure described by the manufacturer.
|
| Scan protocol |
Hybridization signals were detected using the Genepix 4000B Scanner (Molecular Devices, Sunnyvale, CA).
|
| Description |
miRNA expression profile of surgically resected material
|
| Data processing |
Scanned images were analyzed using the Array-Pro Analyzer ver.4.5 software (Media Cybernetics, Rockville, MD). Because two different probe desigins (lot numbers 31015 and 31078) were used, the common probes between them were extracted. The obtained miRNA microarray datasets were then normalized by the quantile normalization method using R statistical software version 2.12.1.
|
| |
|
| Submission date |
Mar 14, 2013 |
| Last update date |
May 15, 2014 |
| Contact name |
Kaoru Mogushi |
| E-mail(s) |
mogushi-k@umin.ac.jp
|
| Phone |
+81-3-5802-1797
|
| Organization name |
Juntendo University
|
| Department |
Intractable Disease Research Center
|
| Street address |
2-1-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8421 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16794 |
| Series (1) |
| GSE45179 |
Intrahepatic miRNA/mRNA expression in non-responders to pegylated interferon plus ribavirin combination therapy for chronic hepatitis C (miRNA microarray data) |
|
