|
| Status |
Public on Mar 19, 2013 |
| Title |
Non-malignant Lymph Node LN3 |
| Sample type |
RNA |
| |
|
| Source name |
lymph node
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Formalin-fixed Paraffin-embedded lymph nodes
histology: non-malignant
|
| Treatment protocol |
Formalin-fixed paraffin-embedded tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using using RecoverAll™ Total Nucleic Acid Isolation kit (Ambion) as per manufacturer's instructions
|
| Label |
Cyanine 3-pcp
|
| Label protocol |
100ng RNA were labelled with Cyanine-3cpc using the agilent miRNA complete labelling and Hyb kit
|
| |
|
| Hybridization protocol |
Labelled RNA was hybridised using agilent miRNA complete labelling and Hyb kit
|
| Scan protocol |
Scanned using Agilent Technologies Scanner G2505C US80603148
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) to obtain background subtracted and spatially detrended. Processed Signal intensities Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Mar 18, 2013 |
| Last update date |
Mar 19, 2013 |
| Contact name |
Kimberley Jones |
| E-mail(s) |
kimberley.jones@qimr.edu.au
|
| Organization name |
Queensland Institute of Medical Research
|
| Street address |
300 Herston Road
|
| City |
Brisbane |
| State/province |
QLD |
| ZIP/Postal code |
4101 |
| Country |
Australia |
| |
|
| Platform ID |
GPL16770 |
| Series (1) |
| GSE45264 |
miRNA expression in classical Hodgkin lymphoma diseased nodes |
|
