|
Status |
Public on Mar 19, 2013 |
Title |
cHL diseased node MC7 |
Sample type |
RNA |
|
|
Source name |
cHL diseased node
|
Organism |
Homo sapiens |
Characteristics |
tissue: Formalin-fixed Paraffin-embedded cHL diseased node histology: Mixed Cellularity cHL
|
Treatment protocol |
Formalin-fixed paraffin-embedded tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using using RecoverAllâ„¢ Total Nucleic Acid Isolation kit (Ambion) as per manufacturer's instructions
|
Label |
Cyanine 3-pcp
|
Label protocol |
100ng RNA were labelled with Cyanine-3cpc using the agilent miRNA complete labelling and Hyb kit
|
|
|
Hybridization protocol |
Labelled RNA was hybridised using agilent miRNA complete labelling and Hyb kit
|
Scan protocol |
Scanned using Agilent Technologies Scanner G2505C US80603148
|
Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) to obtain background subtracted and spatially detrended. Processed Signal intensities Non-uniform outliers were excluded.
|
|
|
Submission date |
Mar 18, 2013 |
Last update date |
Mar 19, 2013 |
Contact name |
Kimberley Jones |
E-mail(s) |
kimberley.jones@qimr.edu.au
|
Organization name |
Queensland Institute of Medical Research
|
Street address |
300 Herston Road
|
City |
Brisbane |
State/province |
QLD |
ZIP/Postal code |
4101 |
Country |
Australia |
|
|
Platform ID |
GPL16770 |
Series (1) |
GSE45264 |
miRNA expression in classical Hodgkin lymphoma diseased nodes |
|