Total RNA was extracted using the TRIzol Reagent method according to the manufacturer’s protocol (Invitrogen) and quantified using Bioanalyzer (Agilent Technologies). Total RNA from normal human osteoblasts was retrieved as described previously (Sadikovic B, Yoshimoto M, Chilton-MacNeill S, et al. Identification of interactive networks of gene expression associated with osteosarcoma oncogenesis by integrated molecular profiling. Hum Mol Genet 2009;18:1962-75).
Label
biotin
Label protocol
See Geiss GK, Bumgarner RE, Birditt B, et al. Direct multiplexed measurement of gene expression with color-coded probe pairs. Nat Biotechnol 2008;26:317-25.
Hybridization protocol
See Geiss GK, Bumgarner RE, Birditt B, et al. Direct multiplexed measurement of gene expression with color-coded probe pairs. Nat Biotechnol 2008;26:317-25.
Scan protocol
See Geiss GK, Bumgarner RE, Birditt B, et al. Direct multiplexed measurement of gene expression with color-coded probe pairs. Nat Biotechnol 2008;26:317-25.
Description
SAMPLE 5 Replicate 2
Data processing
Followed standard nCounter™ Data Analysis Guidelines (linked as supplementary PDF file)
