|
Status |
Public on Mar 22, 2013 |
Title |
ipk1: 251632210288-5 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
mid-log_YEPD
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: ACY044 genotype/variation: wild type
|
Extracted molecule |
total RNA |
Extraction protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
Label |
cy3
|
Label protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
|
|
Channel 2 |
Source name |
mid-log_YEPD
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: ACY237 genotype/variation: ipk1 mutant
|
Extracted molecule |
total RNA |
Extraction protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
Label |
cy5
|
Label protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
|
|
|
Hybridization protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
Scan protocol |
scanned on genepix 4000b
|
Description |
Sample 23
|
Data processing |
images were quantified with genepix pro software version 6.1. background subtraction, loess normalization, and primary filtering performed using stanford microarray database version 2.14 (Nucl. Acids Res. (2007) 35 (suppl 1): D766-D770. doi: 10.1093/nar/gkl1019). Secondary filtering using Cluster 3.0. Filtered: 1) regression correlation >0.6 AND mean intensity/median background intensity is >1.5-fold in EITHER channel. 2) at least 1 observation with abs(val) >=1.6 (3-fold) normalized log2 ratio (cy5/cy3). Unless otherwise stated (in the Description column), arrays are test/reference.
|
|
|
Submission date |
Mar 20, 2013 |
Last update date |
Mar 22, 2013 |
Contact name |
jeremy worley |
Organization name |
university of arizona
|
Department |
MCB
|
Lab |
Capaldi
|
Street address |
1007 e. lowell st.
|
City |
tucson |
State/province |
az |
ZIP/Postal code |
85721 |
Country |
USA |
|
|
Platform ID |
GPL16244 |
Series (1) |
GSE45370 |
Role of pp-ips in rpd3 complex and the environmental stress response (ESR) |
|