|
| Status |
Public on Mar 22, 2013 |
| Title |
kcs1/vip1+Osmotic Stress v. kcs1/vip1: 251632210462-5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mid-log_YEPD
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: ACY236
genotype/variation: kcs1/vip1 mutant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| Label |
cy3
|
| Label protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| |
|
| Channel 2 |
| Source name |
KCl stress 0.375M_20min
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: ACY236
genotype/variation: kcs1/vip1 mutant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| Label |
cy5
|
| Label protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| |
|
| |
| Hybridization protocol |
as described in capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| Scan protocol |
scanned on genepix 4000b
|
| Description |
Sample 35
|
| Data processing |
images were quantified with genepix pro software version 6.1.
background subtraction, loess normalization, and primary filtering performed using stanford microarray database version 2.14 (Nucl. Acids Res. (2007) 35 (suppl 1): D766-D770. doi: 10.1093/nar/gkl1019). Secondary filtering using Cluster 3.0. Filtered: 1) regression correlation >0.6 AND mean intensity/median background intensity is >1.5-fold in EITHER channel. 2) at least 1 observation with abs(val) >=1.6 (3-fold)
normalized log2 ratio (cy5/cy3). Unless otherwise stated (in the Description column), arrays are test/reference.
|
| |
|
| Submission date |
Mar 20, 2013 |
| Last update date |
Mar 22, 2013 |
| Contact name |
jeremy worley |
| Organization name |
university of arizona
|
| Department |
MCB
|
| Lab |
Capaldi
|
| Street address |
1007 e. lowell st.
|
| City |
tucson |
| State/province |
az |
| ZIP/Postal code |
85721 |
| Country |
USA |
| |
|
| Platform ID |
GPL16244 |
| Series (1) |
| GSE45370 |
Role of pp-ips in rpd3 complex and the environmental stress response (ESR) |
|
