|
| Status |
Public on Dec 04, 2013 |
| Title |
8_ganglion-number_10 |
| Sample type |
SRA |
| |
|
| Source name |
Ganglion 10
|
| Organism |
Hirudo medicinalis |
| Characteristics |
tissue: Ganglia chain
age: adult
|
| Growth protocol |
All were obtained from a Hirudo Medicinalis colony grown in France at Ricarimpex Farm and maintained in our lab. Prior to use, all grew under the same environment (tanks populated with about 20 leeches, temp of 16ºc).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Lipid Tissue(Qiagen)
illumina hiseq preparation kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
8_ganglion-number_10_ACAGTG_L008_R1_001.fastq
|
| Data processing |
FastQc for quality conroll
Trans ABySS for de novo transcriptome assembly with SE parameters
Trinity for de novo transcriptome assembly with inchworm method
RSEM for alignment and estimating counts
Tophat for alignment
cufflinks for estimate expression
Genome_build: n/a (de novo assembly)
|
| |
|
| Submission date |
Mar 28, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Dror Hibsh |
| Organization name |
Bar Ilan University
|
| Department |
Bioinformatics
|
| Street address |
Bar Ilan
|
| City |
Ramat Gan |
| ZIP/Postal code |
52900 |
| Country |
Israel |
| |
|
| Platform ID |
GPL16891 |
| Series (1) |
| GSE45569 |
Spatial Regulation Dominates Gene Function in the Ganglia Chain |
|
| Relations |
| SRA |
SRX256705 |
| BioSample |
SAMN01993653 |
