|
| Status |
Public on Mar 29, 2013 |
| Title |
IFNAR_Tg_1 |
| Sample type |
RNA |
| |
|
| Source name |
IFNAR2-/- BMMC, 17 hours post-infection, Toxoplasma
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
cell type: macrophage
genotype/variation: IFNAR2-/-
infection: Toxoplasma
|
| Treatment protocol |
RNA was immediately isolated from the cells 17 hours after infection.
|
| Growth protocol |
Bone marrow collected from femurs and tibias of WT or IFNAR2-/- mice was cultured in petri dishes for 5-6 days in DMEM media supplemented with antibiotics, 1mM non-essential amino acids, 1mM sodium pyruvate, 15% fetal calf serum, and 25% conditioned supernatant from L929 cells (source of M-CSF) in order to differentiate cells to macrophages. After differentiation, cells were collected by scraping and were replated in tissue culture-treated 6-well plates. Cells were allowed to adhere overnight, then were infected with a 1:1 MOI of either Toxoplasma or Neospora. 17 hours after infection, RNA was isolated from cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed by treatement with Buffer RLT (Qiagen) and passage over QiaShredder columns (Qiagen). Total RNA was isolated using the Qiagen Rneasy Plus kit.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled complementary RNA (cRNA) was made using the Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
infected
|
| Data processing |
The data were quantile normalized without background subtraction using Illumina GenomeStudio V1.8
|
| |
|
| Submission date |
Mar 29, 2013 |
| Last update date |
Mar 29, 2013 |
| Contact name |
daniel beiting |
| E-mail(s) |
beiting@vet.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Pathobiology
|
| Lab |
Beiting
|
| Street address |
380 S. University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE45632 |
Differential induction of TLR3-dependent innate immune signaling by closely related parasite species_I |
| GSE45634 |
Differential induction of TLR3-dependent innate immune signaling by closely related parasite species |
|
