|
| Status |
Public on Apr 08, 2014 |
| Title |
SQ20B 0.01% DMSO 48 hr-replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
SQ20B cultured 48 hr 0.01% DMSO (veh)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SQ20B
cell type: Larynx, squamous cell carcinoma cells
treated with: 0.01% DMSO (veh) for 48h
|
| Biomaterial provider |
a gift from Dr. Anjali Gupta (Dept of Radiation Oncology, The Univ. of Iowa)
|
| Treatment protocol |
To the cell lines either 5uM Erlotinib in DMSO or DMSO (vehicle) was added 48 hours prior to RNA harvest (100,000 cells cultured for 48 hr + 0.01%DMSO (veh) or + 5uM erlotinib in DMSO)
|
| Growth protocol |
Cell lines were maintained in DMEM (11965-092 Gibco) media containing 10% Hyclone Fetal Bovine Serum and supplemented with 5mM Sodium Pyruvate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina Hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
02_SQ20B-2
|
| Data processing |
The data was quantile normalized in Genome Studio (v2011.1).
|
| |
|
| Submission date |
Apr 09, 2013 |
| Last update date |
Apr 08, 2014 |
| Contact name |
Thomas B Bair |
| E-mail(s) |
iihg-bioinformatics@uiowa.edu
|
| Organization name |
University of Iowa
|
| Street address |
335 EMRB
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE45891 |
Genome-wide analysis of EGFR inhibitor erlotinib treatment in Head and neck, squamous cell carcinoma's FADU, SQ20B and Cal 27 |
|
