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Sample GSM1124247 Query DataSets for GSM1124247
Status Public on Apr 20, 2013
Title M_133A_3_AnCg_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Anterior Cingulate
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133P
subject id: 3
brain region: anterior cingulate
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.59037607
10001_at 7.131453721
10002_at 5.433773192
10003_at 5.149851731
100048912_at 6.106788346
10004_at 5.470246375
10005_at 7.650382771
10006_at 9.391743493
10007_at 8.63684852
10009_at 5.0059075
1000_at 8.835798523
10010_at 6.486700081
100127886_at 4.708171438
100127972_at 5.124045864
100128008_at 7.524311749
100128062_at 9.840728886
100128414_at 4.849252295
100128919_at 6.989809249
100129015_at 5.198800473
100129128_at 5.219020117

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124247_M_133A_3_AnCg_1_C_p.cel.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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