|
| Status |
Public on Dec 09, 2013 |
| Title |
eMCAo 48hrs |
| Sample type |
RNA |
| |
|
| Source name |
ischemic brain
|
| Organism |
Rattus norvegicus |
| Characteristics |
species: Wistar rat
gender: male
weight: 280-320g
tissue: ischemic brain
|
| Treatment protocol |
no treatment
|
| Growth protocol |
All animals were handled according to the Council for International Organisation of Medical Sciences on Animal Experimentation (World Health Organisation, Geneva, Switzerland) and the National University of Singapore (IACUC/NUS) guidelines for laboratory animals. The protocol was approved by the Committee on the Ethics of Animal Experiments of the National University of Singapore (Protocol Number: 081/09 and 025/11). All surgery was performed using ketamine/xylazine as anesthesia, and all efforts were made to minimize suffering.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (+ miRNA) was extracted from brain tissues by a single-step method using Trizol reagent (Invitrogen, Life Technologies, USA) according to manufacturers’ protocol.
|
| Label |
biotin
|
| Label protocol |
Biotin-labelled complementary RNA probes were synthesized with the Illumina TotalPrep RNA Amplification kit (Ambion).
|
| |
|
| Hybridization protocol |
Hybridization was performed in 15ul volume by using illumina hybridization kit.
|
| Scan protocol |
Data were scanned and analysed with the BeadStudio software (v.1.8.0).
|
| Description |
5320724053_D.AVG_Signal
400ng of total RNA was detected by beadchip
|
| Data processing |
Differentially expressed genes were selected based on p value < 0.05, differential score > 20, average signal intensity > 100, and normalize to endo-control and baseline (Illumina Inc. GenomeStudio Version 1.8.0).
|
| |
|
| Submission date |
Apr 22, 2013 |
| Last update date |
Dec 09, 2013 |
| Contact name |
Kandiah Jeyaseelan |
| E-mail(s) |
erijeya@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Biochemistry
|
| Street address |
8 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117597 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6101 |
| Series (2) |
| GSE46267 |
microRNAs involved in regulating embolic stroke recovery following spontaneous reperfusion [mRNA] |
| GSE46269 |
miRNAs involved in regulating embolic stroke recovery following spontaneous reperfusion |
|
