|
| Status |
Public on Apr 25, 2013 |
| Title |
Stems of D11 mutant |
| Sample type |
RNA |
| |
|
| Source name |
Stems_mut
|
| Organism |
Zea mays |
| Characteristics |
tissue: Stem
genotype: D11 mutant
developmental stage: Tassel emergence
|
| Treatment protocol |
No
|
| Growth protocol |
All experimental materials were single-grain sowed and nursed with normal agricultural practice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Description |
Gene expression data from D11
|
| Data processing |
Raw data were normalized by MAS 5.0 algorithm implemented in Expression Console.
|
| |
|
| Submission date |
Apr 25, 2013 |
| Last update date |
Apr 25, 2013 |
| Contact name |
Yijun Wang |
| E-mail(s) |
wyj@yzu.edu.cn
|
| Organization name |
Yangzhou University
|
| Street address |
Wenhui
|
| City |
Yangzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
225009 |
| Country |
China |
| |
|
| Platform ID |
GPL4032 |
| Series (1) |
| GSE46370 |
Expression data from maize D11 mutant |
|
