|
| Status |
Public on Jun 20, 2014 |
| Title |
CD4T-cells_C57BL/6 mice_3wk_rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
CD4T-cells purified from spleen leukocytes collected from 3 wk-old C57BL/6 mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: untreated Spleen CD4T-Cells
strain: C57BL/6 mice
age: 3 week
|
| Treatment protocol |
CD4 T-cellswere untreated
|
| Growth protocol |
CD4 T-cells were uncultured
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-1.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix microArrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from untreated spleen CD4 T-cells of 3 week-old mice.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 02, 2013 |
| Last update date |
Jun 20, 2014 |
| Contact name |
Dorothy N. Kakoola |
| E-mail(s) |
dkakoola@uthsc.edu
|
| Organization name |
University of Tennessee Health Science Center
|
| Department |
Medicine
|
| Lab |
VA Research
|
| Street address |
1030 Jefferson Avenue
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE46600 |
Transcriptome and Molecular Pathways Analysis of CD4 T-Cells from Young NOD Mice |
|
