|
| Status |
Public on Jan 10, 2014 |
| Title |
patient 60 normal tissue no smoking yes obese Par004U133Plus2.0.CEL |
| Sample type |
RNA |
| |
|
| Source name |
kidney tissue
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 60
tissue: normal
smoking: no
obese: yes
batch: 1
duplicate: no
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The Mayo Biospecimen Accessioning and Processing Core performed RNA extractions using kits and protocols from the Qiagen miRNEasy kit and Qiagen Qiacube instrument.
|
| Label |
biotin
|
| Label protocol |
Microarray analysis was conducted according to manufacturer’s instructions for the Affymetrix One Cycle Target Labeling and Control Reagents kit
|
| |
|
| Hybridization protocol |
The products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA. The IVT products were then column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h. Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin
|
| Scan protocol |
Arrays were scanned in an Affymetrix GeneChip® Scanner 3000
|
| Data processing |
Base-2 logarithm transformed intensity data from the two batches of samples were normalized within each batch using frozen robust multi-array analysis (frozen RMA).
|
| |
|
| Submission date |
May 07, 2013 |
| Last update date |
Jan 11, 2014 |
| Contact name |
Daniel Serie |
| E-mail(s) |
serie.daniel@mayo.edu
|
| Phone |
9049536451
|
| Organization name |
Mayo Clinic
|
| Street address |
4500 San Pablo Rd
|
| City |
Jacksonville |
| State/province |
Florida |
| ZIP/Postal code |
32224 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE46699 |
Smoking and Obesity Related Molecular Alterations in Clear Cell Renal Cell Carcinoma |
|
