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Sample GSM114036 Query DataSets for GSM114036
Status Public on Nov 21, 2006
Title large airways, non-smoker 010316L, MAS5
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 24
Sex: M
Ethnic group: black
Smoking Status: non-smoker
Lung: left
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HuGeneFL. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneArray 2500.
Description large airways, non-smoker 010316L, MAS5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 12, 2006
Last update date Nov 21, 2006
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL80
Series (2)
GSE5056 Airway epithelium, large airways, phenotypically normal smokers vs non-smokers, MAS5 (HuGeneFL)
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD

Data table header descriptions
ID_REF Probe set ID
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
A28102_at 927.4 A 0.262827
AB000114_at 231.6 A 0.9273
AB000115_at 176.2 A 0.712257
AB000220_at 566.5 A 0.195266
AB000381_s_at 179.2 A 0.794268
AB000409_at 115.9 A 0.945787
AB000410_s_at 416.3 A 0.783476
AB000449_at 747.2 A 0.239063
AB000450_at 634.9 A 0.354453
AB000460_at 2324.8 P 0.010317
AB000462_at 86.5 A 0.949771
AB000464_at 561.1 A 0.52976
AB000466_at 265.6 A 0.981719
AB000467_at 584.8 A 0.910522
AB000468_at 2913.4 P 0.012547
AB000584_at 3734.1 A 0.0727
AB000816_s_at 116.9 A 0.760937
AB000895_at 1149.2 A 0.5
AB000896_at 562.5 A 0.645547
AB000897_at 251.3 A 0.860518

Total number of rows: 7129

Table truncated, full table size 197 Kbytes.




Supplementary data files not provided
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