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Sample GSM114038 Query DataSets for GSM114038
Status Public on Nov 21, 2006
Title large airways, non-smoker 010430L, MAS5
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 48
Sex: M
Ethnic group: black
Smoking Status: non-smoker
Lung: left
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HuGeneFL. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneArray 2500.
Description large airways, non-smoker 010430L, MAS5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 12, 2006
Last update date Nov 21, 2006
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL80
Series (2)
GSE5056 Airway epithelium, large airways, phenotypically normal smokers vs non-smokers, MAS5 (HuGeneFL)
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD

Data table header descriptions
ID_REF Probe set ID
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
A28102_at 1811.4 A 0.147939
AB000114_at 409.8 A 0.760937
AB000115_at 2263.2 P 0.004017
AB000220_at 1703.8 P 0.00011
AB000381_s_at 301.5 A 0.883887
AB000409_at 443.2 A 0.81489
AB000410_s_at 257.3 A 0.937071
AB000449_at 311.2 A 0.659339
AB000450_at 93.7 A 0.941556
AB000460_at 2248 P 0.013804
AB000462_at 884.8 A 0.440646
AB000464_at 774.5 A 0.41138
AB000466_at 308.6 A 0.997977
AB000467_at 454.8 A 0.921998
AB000468_at 2080.5 P 0.028457
AB000584_at 4057 A 0.095667
AB000816_s_at 832.8 A 0.672921
AB000895_at 1510.6 A 0.440646
AB000896_at 219 A 0.921998
AB000897_at 1037.2 A 0.300606

Total number of rows: 7129

Table truncated, full table size 197 Kbytes.




Supplementary data files not provided
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