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Sample GSM114046 Query DataSets for GSM114046
Status Public on Nov 21, 2006
Title large airways, non-smoker 011128L, MAS5
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 39
Sex: M
Ethnic group: black
Smoking Status: non-smoker
Lung: left
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HuGeneFL. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneArray 2500.
Description large airways, non-smoker 011128L, MAS5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 12, 2006
Last update date Nov 21, 2006
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL80
Series (2)
GSE5056 Airway epithelium, large airways, phenotypically normal smokers vs non-smokers, MAS5 (HuGeneFL)
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD

Data table header descriptions
ID_REF Probe set ID
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
A28102_at 1233.1 P 0.018281
AB000114_at 638.9 P 0.000225
AB000115_at 1448 P 0.003212
AB000220_at 5789.3 P 0.000044
AB000381_s_at 233 A 0.455413
AB000409_at 2131.2 P 0.004017
AB000410_s_at 255.6 A 0.737173
AB000449_at 1832.5 P 0.000044
AB000450_at 1622.4 P 0.001593
AB000460_at 4783.3 P 0.000147
AB000462_at 1079.6 P 0.01667
AB000464_at 1866.2 P 0.005565
AB000466_at 414.4 A 0.876428
AB000467_at 1606.1 A 0.455413
AB000468_at 4181.5 P 0.000147
AB000584_at 6419.8 P 0.010317
AB000816_s_at 38.1 A 0.891021
AB000895_at 418 A 0.724854
AB000896_at 1653.9 A 0.095667
AB000897_at 1001.9 P 0.028457

Total number of rows: 7129

Table truncated, full table size 196 Kbytes.




Supplementary data files not provided
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