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Sample GSM114076 Query DataSets for GSM114076
Status Public on Nov 21, 2006
Title large airways, smoker 020506L, MAS5
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 41
Sex: M
Ethnic group: black
Smoking Status: smoker, 20 pack-years
Lung: left
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HuGeneFL. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneArray 2500.
Description large airways, non-smoker 020506L, MAS5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 12, 2006
Last update date Dec 19, 2006
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL80
Series (2)
GSE5056 Airway epithelium, large airways, phenotypically normal smokers vs non-smokers, MAS5 (HuGeneFL)
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD

Data table header descriptions
ID_REF Probe set ID
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
A28102_at 736.2 M 0.050229
AB000114_at 237.7 A 0.095667
AB000115_at 783.9 P 0.004484
AB000220_at 3779.8 P 0.000052
AB000381_s_at 164.8 A 0.470241
AB000409_at 1881.8 P 0.002023
AB000410_s_at 252.2 A 0.834139
AB000449_at 404.6 P 0.003212
AB000450_at 1124.3 P 0.00762
AB000460_at 4664.3 P 0.000258
AB000462_at 436.3 A 0.123572
AB000464_at 1053.2 P 0.018281
AB000466_at 240.2 A 0.978098
AB000467_at 832.5 A 0.737173
AB000468_at 6752.8 P 0.002556
AB000584_at 5511.4 P 0.021902
AB000816_s_at 37.4 A 0.737173
AB000895_at 414.7 A 0.659339
AB000896_at 844.2 A 0.275146
AB000897_at 297.9 A 0.39692

Total number of rows: 7129

Table truncated, full table size 196 Kbytes.




Supplementary data files not provided
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