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Sample GSM114094 Query DataSets for GSM114094
Status Public on Nov 21, 2006
Title small airways, COPD 015, MAS5
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 52
Sex: M
Ethnic group: white
Smoking Status: COPD, GOLD-II, 18 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G
Description small airways, COPD 015, MAS5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 12, 2006
Last update date Aug 28, 2018
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (4)
GSE5058 Airway epithelium, small airways, normal non-smokers, phenotypic normal smokers, smokers with COPD and early COPD
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD
GSE8545 Variability in Small Airway Epithelial Gene Expression Among Normal Smokers
Relations
Reanalyzed by GSE60486
Reanalyzed by GSE119087

Data table header descriptions
ID_REF Probe set ID
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 6792.3 P 0.000219
1053_at 431.8 P 0.017001
117_at 140.5 A 0.081337
121_at 488.3 P 0.035163
1255_g_at 19.5 A 0.5
1294_at 795 P 0.002228
1316_at 207.8 P 0.02786
1320_at 9.2 A 0.988553
1405_i_at 1955.4 P 0.000491
1431_at 85.8 P 0.019304
1438_at 223.2 P 0.000805
1487_at 663.3 A 0.107301
1494_f_at 518 P 0.002617
1552256_a_at 265.3 P 0.023926
1552257_a_at 1368.9 P 0.000244
1552258_at 135.5 A 0.19458
1552261_at 388.9 P 0.005859
1552263_at 415.5 P 0.000244
1552264_a_at 537.9 P 0.010742
1552266_at 102.6 P 0.018555

Total number of rows: 54675

Table truncated, full table size 1445 Kbytes.




Supplementary file Size Download File type/resource
GSM114094.CEL.gz 8.4 Mb (ftp)(http) CEL
GSM114094.CHP.gz 13.3 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
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