|
Status |
Public on Jan 01, 2016 |
Title |
SCC13 miR-203 overexpressing cells, 13_203_2 |
Sample type |
RNA |
|
|
Source name |
SCC13 human cell line, miR-203
|
Organism |
Homo sapiens |
Characteristics |
cell line: SCC13 stable infection: miR-203 batch infection: 1 technical replicate: 2
|
Treatment protocol |
Total RNA (including small RNAs) was isolated from cultured cells stably infected in biological duplicate with either a scrambled control hairpin or miR-203. Samples were harvested in technical duplicate.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using a miRNeasy Mini Kit (Qiagen) and RNA quality was analyzed using a Bioanalyzer 2100 (Agilent) and RNA Nano Chip. RIN values were all above 9.3.
|
Label |
Biotin
|
Label protocol |
Standard Illumina labeling protocol.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
Established squamous cell carcinoma cell line from facial SCC.
|
Data processing |
The data was log base 2 quantile normalised after artifact removal using the BASH and HULK algorithms in the R beadarray package.
|
|
|
Submission date |
May 16, 2013 |
Last update date |
Jan 01, 2016 |
Contact name |
Nathan Benaich |
Organization name |
King's College London
|
Department |
Centre for Stem Cells and Regenerative Medicine
|
Lab |
28th Floor, Tower Wing, Guy's Hospital
|
Street address |
Great Maze Pond
|
City |
London |
ZIP/Postal code |
SE1 9RT |
Country |
United Kingdom |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE47028 |
miR-203, a microRNA of multilayered epithelia, inhibits metastatic fitness in human squamous cell carcinoma |
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