|
| Status |
Public on Jan 01, 2016 |
| Title |
SJG15 control cells, 15_EV_2 |
| Sample type |
RNA |
| |
|
| Source name |
SJG15 human cell line, control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SJG15
stable infection: scrambled control hairpin
batch infection: 1
technical replicate: 2
|
| Treatment protocol |
Total RNA (including small RNAs) was isolated from cultured cells stably infected in biological duplicate with either a scrambled control hairpin or miR-203. Samples were harvested in technical duplicate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a miRNeasy Mini Kit (Qiagen) and RNA quality was analyzed using a Bioanalyzer 2100 (Agilent) and RNA Nano Chip. RIN values were all above 9.3.
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina labeling protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Primary, early passage squamous cell carcinoma cell line from tongue SCC.
|
| Data processing |
The data was log base 2 quantile normalised after artifact removal using the BASH and HULK algorithms in the R beadarray package.
|
| |
|
| Submission date |
May 16, 2013 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Nathan Benaich |
| Organization name |
King's College London
|
| Department |
Centre for Stem Cells and Regenerative Medicine
|
| Lab |
28th Floor, Tower Wing, Guy's Hospital
|
| Street address |
Great Maze Pond
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE47028 |
miR-203, a microRNA of multilayered epithelia, inhibits metastatic fitness in human squamous cell carcinoma |
|
