|
Status |
Public on Sep 18, 2013 |
Title |
RNA (diagnosis) from BCP-ALL patient ALL_369 |
Sample type |
RNA |
|
|
Source name |
Bone marrow
|
Organism |
Homo sapiens |
Characteristics |
tissue: Bone marrow cell type: primary ALL (acute lymphoblastic leukemia) cells at diagnosis tumor subtype: t(9;22)
|
Treatment protocol |
Ficoll-isopaque centrifugation
|
Growth protocol |
NA, primary cells
|
Extracted molecule |
total RNA |
Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared from two micrograms of total RNA
|
|
|
Hybridization protocol |
Biotinylated cRNAs were hybridized to the gene chips for 16 hours in a 45°C incubator, rotated at 60 rpm.
|
Scan protocol |
The GeneChips were then washed and stained using the Fluidics Station 450 and scanned using the GeneChip® Scanner 3000 7G
|
Description |
re-analysis of GSM661395
|
Data processing |
The data was normalized using the robust multi-array average (RMA) method (Irizarry et al. 2003)
|
|
|
Submission date |
May 17, 2013 |
Last update date |
Nov 14, 2018 |
Contact name |
Jessica Nordlund |
E-mail(s) |
jessica.nordlund@medsci.uu.se
|
Phone |
+46186114912
|
Organization name |
Uppsala University
|
Department |
Medical Sciences
|
Lab |
Molecular Medicine
|
Street address |
Academic Hospital, Entrance 70, 3rd floor
|
City |
Uppsala |
ZIP/Postal code |
75185 |
Country |
Sweden |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE47051 |
Genome-wide differential gene expression signatures in pediatric acute lymphoblastic leukemia subtypes |
GSE49032 |
Genome-wide signatures of differential DNA methylation and gene expression in pediatric ALL |
|
Relations |
Reanalysis of |
GSM661395 |
Reanalyzed by |
GSE122511 |