|
| Status |
Public on Sep 18, 2013 |
| Title |
RNA (diagnosis) from BCP-ALL patient ALL_369 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow
cell type: primary ALL (acute lymphoblastic leukemia) cells at diagnosis
tumor subtype: t(9;22)
|
| Treatment protocol |
Ficoll-isopaque centrifugation
|
| Growth protocol |
NA, primary cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from two micrograms of total RNA
|
| |
|
| Hybridization protocol |
Biotinylated cRNAs were hybridized to the gene chips for 16 hours in a 45°C incubator, rotated at 60 rpm.
|
| Scan protocol |
The GeneChips were then washed and stained using the Fluidics Station 450 and scanned using the GeneChip® Scanner 3000 7G
|
| Description |
re-analysis of GSM661395
|
| Data processing |
The data was normalized using the robust multi-array average (RMA) method (Irizarry et al. 2003)
|
| |
|
| Submission date |
May 17, 2013 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Jessica Nordlund |
| E-mail(s) |
jessica.nordlund@medsci.uu.se
|
| Phone |
+46186114912
|
| Organization name |
Uppsala University
|
| Department |
Medical Sciences
|
| Lab |
Molecular Medicine
|
| Street address |
Academic Hospital, Entrance 70, 3rd floor
|
| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE47051 |
Genome-wide differential gene expression signatures in pediatric acute lymphoblastic leukemia subtypes |
| GSE49032 |
Genome-wide signatures of differential DNA methylation and gene expression in pediatric ALL |
|
| Relations |
| Reanalysis of |
GSM661395 |
| Reanalyzed by |
GSE122511 |
