|
| Status |
Public on May 09, 2016 |
| Title |
genotype: WT - drought: CTRL - rep3 |
| Sample type |
RNA |
| |
|
| Source name |
flag leaf (genotype: WT - drought: CTRL)
|
| Organism |
Triticum turgidum |
| Characteristics |
germplasm name: Borgia
organism part: flag leaf ('PO:0020103')
development stage: 4 anthesis ('PO:0007616')
growth media: not specified
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy Plant Mini Kit
|
| Label |
biotin
|
| Label protocol |
Affymetrix one-cycle target labeling
|
| |
|
| Hybridization protocol |
Affymetrix Basic Protocol
|
| Scan protocol |
Affy- Array Scanning
|
| Description |
WT: Borgia.
CTRL: the soil was maintained at its maximum water holding capacity during the whole experiment. (May still include original submitter created erros/discrepancies)
|
| Data processing |
The MAS5.0 Normalization was performed using the justMAS function in the simpleaffy package of Bioconductor (www.bioconductor.org, Version: 2.12.1). The target value is 500 for all cases. The code was developed using R2.4.1 to 2.13.0, Bioconductor 1.9 to 2.12.1, and simpleaffy 2.8.0. to 2.28.0 The CDF files were downloaded from the Bioconductor website at http://www.bioconductor.org/packages/release/AffymetrixChip.html.
|
| |
|
| Submission date |
May 20, 2013 |
| Last update date |
May 09, 2016 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
|
| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platform ID |
GPL3802 |
| Series (1) |
| GSE47090 |
Pale-green durum wheat mutant under terminal drought stress condition |
|
