|
Status |
Public on May 21, 2013 |
Title |
Pooled sample at d0, left plantaris, young mice |
Sample type |
RNA |
|
|
Source name |
Left plantaris muscle, d0
|
Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6J gender: male tissue: skeletal muscle (left plantaris) age: 5 months time point: day 0
|
Growth protocol |
Male C57BL/6J mice (The Jackson Laboratory, Bar Harbor, ME), 5 months of age were housed in a temperature- and humidity-controlled facility on a 14:10 h light:dark cycle with access to food and water ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cDNA were prepared according to the standard Ambion and Affymetrix protocol from 250 ng total RNA (Ambion WT Expression kit and Affymetrix WT Terminal Labeling kit).
|
|
|
Hybridization protocol |
Following fragmentation, 2.5 ug of cDNA were hybridized for 17 hr at 45C on GeneChip Mouse Gene 1.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 7G Scanner.
|
Description |
Overload d0, left Gene expression data from left plantaris muscle, d0
|
Data processing |
The CEL data were generated using Affymetrix Command Console software and the data analysis was performed using Partek Genomics Suite.
|
|
|
Submission date |
May 20, 2013 |
Last update date |
May 21, 2013 |
Contact name |
Thomas Chaillou |
E-mail(s) |
thomas.chaillou@uky.edu
|
Organization name |
Department of Physiology
|
Street address |
University of Kentucky, Department of Physiology, 800 Rose Street
|
City |
Lexington |
State/province |
KY |
ZIP/Postal code |
40536-0298 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE47098 |
Expression data during plantaris muscle hypertrophy induced by synergist ablation in young adult mice |
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