|
Status |
Public on Apr 21, 2014 |
Title |
shNS, Glucagon B |
Sample type |
RNA |
|
|
Source name |
Primary hepatocytes, transduced for 72 hours with non-specific shRNA. Ttreated with 100nM glucagon for 90 minutes.
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL6/J gender: Male age: 10 weeks cell type: Primary hepatocytes
|
Treatment protocol |
Primary hepatocytes were transduced with non-specific shRNA, shKAT2B or shWDR5 (2-4 pfu/cell) and cultured for 72 hours. Cells treated with 100nM glucagon or PBS for 90 min.
|
Growth protocol |
Primary hepatocytes were grown in Medium 199 from Mediatech supplemented with 100mg/ml Penicillin/Streptomycin. Medium was changed daily.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol followed by the Qiagen RNAeasy kit according to manufacturer's instructions. Each sample contains RNA from three separate culture dishes.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol
|
|
|
Hybridization protocol |
17 hours at 45 degrees
|
Scan protocol |
Chips were scanned using an Affymetrix genechip scanner 3000 7G
|
Description |
shNS, 100nM Glucagon, 90min.
|
Data processing |
Data was processed using the Affymetrix Expression Console software v1.1.1.
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|
|
Submission date |
May 22, 2013 |
Last update date |
Apr 21, 2014 |
Contact name |
Kim Ravnskjaer |
Organization name |
University of Southern Denmark
|
Department |
Department of Biochemistry and Molecular Biology
|
Street address |
Campusvej 55
|
City |
Odense M |
State/province |
CA |
ZIP/Postal code |
5230 |
Country |
Denmark |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE47179 |
Effects of KAT2B and WDR5 depletion on hepatocyte gene expression |
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