|
| Status |
Public on Apr 21, 2014 |
| Title |
shWDR5, Glucagon A |
| Sample type |
RNA |
| |
|
| Source name |
Primary hepatocytes, transduced for 72 hours with WDR5-specific shRNA. Ttreated with 100nM glucagon for 90 minutes.
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
gender: Male
age: 10 weeks
cell type: Primary hepatocytes
|
| Treatment protocol |
Primary hepatocytes were transduced with non-specific shRNA, shKAT2B or shWDR5 (2-4 pfu/cell) and cultured for 72 hours. Cells treated with 100nM glucagon or PBS for 90 min.
|
| Growth protocol |
Primary hepatocytes were grown in Medium 199 from Mediatech supplemented with 100mg/ml Penicillin/Streptomycin. Medium was changed daily.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol followed by the Qiagen RNAeasy kit according to manufacturer's instructions. Each sample contains RNA from three separate culture dishes.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
17 hours at 45 degrees
|
| Scan protocol |
Chips were scanned using an Affymetrix genechip scanner 3000 7G
|
| Description |
shWDR5, 100nM Glucagon, 90min.
|
| Data processing |
Data was processed using the Affymetrix Expression Console software v1.1.1.
|
| |
|
| Submission date |
May 22, 2013 |
| Last update date |
Apr 21, 2014 |
| Contact name |
Kim Ravnskjaer |
| Organization name |
University of Southern Denmark
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Street address |
Campusvej 55
|
| City |
Odense M |
| State/province |
CA |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE47179 |
Effects of KAT2B and WDR5 depletion on hepatocyte gene expression |
|
