|
| Status |
Public on Aug 26, 2013 |
| Title |
ns DHT 2 |
| Sample type |
RNA |
| |
|
| Source name |
24hr non-specific siRNA, 16hr 10nM DHT treatment replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Immortalized AR positive myofibroblast
|
| Treatment protocol |
Plated 2x10e4 cells/well in 6-well dishes for 24h in RPMI 1640 containing 5% hormone striped fetal bovine serum. Cells were transfected with 10nM non-specific siRNA or Hic-5 specific siRNA using LTX-plus transfection reagent in phenol red-free RPMI 1640. Twenty-four hours following transfection, media was replaced with fresh hormone stripped 5% FBS. Fourtyeight hours post transfection cells were treated with 10nM hormone or equivalent vehicle (ethanol)
|
| Growth protocol |
PShTert-AR cells were maintained in RPMI 1640 medium containing 5% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 300ng RNA according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard Affymetrix scanning protocol
|
| Description |
PShTert-AR
|
| Data processing |
Raw data was processed in in R using Bioconductior and Limma. Array data was normalized using RMA, filtered by mapped probes and an arbitary minimum threshold.
|
| |
|
| Submission date |
May 22, 2013 |
| Last update date |
Aug 26, 2013 |
| Contact name |
damien leach |
| E-mail(s) |
damien.leach@adelaide.edu.au
|
| Organization name |
the university of adelaide
|
| Street address |
woodville road
|
| City |
Adelaide |
| ZIP/Postal code |
5011 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE47203 |
Genome-wide expression profiling of Hic-5 knockdown in PshTert-AR human myofibroblasts |
|
