|
| Status |
Public on Nov 26, 2014 |
| Title |
Mammary stem cell enriched basal population, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
mammary stem cell enriched basal population
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype: wildtype
gender: female
developmental stage: adult virgin
tissue: mammary gland
mammary epithelial cell population: mammary stem cells
|
| Treatment protocol |
Mammary glands were minced and digested with enzyme mix to generate single cell suspensions. Cells were sorted by FACSaria (BD).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit (Qiagen, #74134) was used according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Labeling, hybridization and scan performed by team of Dr. E. Fox at the Dana-Farber Cancer Institute Microarray Core Facility. The NuGEN Ovation V2 Amplification System was used to amplify and label the samples, according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The biotinylated cDNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to a microarray chip overnight at 45°C.
|
| Scan protocol |
Each cDNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
|
| Description |
MASC 2
Gene expression data from sorted MASC population, replicate 2
|
| Data processing |
The raw CEL files were imported, normalized and analyzed with GenePattern analysis tools (dCHIP, GSEA) from the Broad Institute.
|
| |
|
| Submission date |
May 25, 2013 |
| Last update date |
Nov 26, 2014 |
| Contact name |
Zhe Li |
| E-mail(s) |
zli4@rics.bwh.harvard.edu
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Medicine, Genetics Division
|
| Lab |
Li Lab
|
| Street address |
77 Avenue Louis Pasteur, Room 466
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE47376 |
Microarray expression profiling of distinct subsets of mouse mammary epithelial cells |
| GSE47377 |
Microarray expression profiling of Runx1-null and wildtype mouse mammary epithelial cells |
|
