|
| Status |
Public on Jul 01, 2014 |
| Title |
1833 control, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB-231 1833 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231 1833
cell type: breast cancer cell line
transduction: control
|
| Treatment protocol |
RKIP expressing cells were generated by transducing the cells with S153E-RKIP expression vector in a lentiviral vector, and were selected and maintained in 0.5 μg/ml puromycin.
|
| Growth protocol |
Cells were cultured in complete medium consisting of DMEM supplemented with 10% FBS, 50 U/ml penicillin and 50 μg/ml streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using miRNeasy Mini Kit following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA Using GeneChip WT terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug cRNA were hybridized on GeneChip Human Genome U133plus2.0 Array using GeneChip Hybridization, Wash and Stain Kit according to manufacturer's protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Using fluidics protocol FS450_0007, and scan with Fluidics Station 450.
|
| Description |
gene expression data from control cells
|
| Data processing |
Hybridization and background correction according to the standard protocols, the quantified signals were normalized using Robust Multi-array Average (RMA).
|
| |
|
| Submission date |
May 25, 2013 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Miao Sun |
| Organization name |
University of Chicago
|
| Street address |
929 E 57th
|
| City |
Chicago |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE47378 |
Gene expression data from 1833 cells expressing RKIP vs control |
| GSE47379 |
1833 cells expressing RKIP vs control |
|
