|
| Status |
Public on Aug 23, 2013 |
| Title |
SK-N-SH 100nM, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
SK-N-SH_100nM_iBET-726
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SK-N-SH
cell type: Neuroblastoma
|
| Treatment protocol |
2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celsius and 5% CO2. The following day, DMSO or iBET-726 were added to final concentrations of 0.1% DMSO or 100 nM/1uM iBET-726. After 16 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celsius.
|
| Growth protocol |
Cells were grown as recommended by ATCC and DSMZ
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
|
| Label |
Biotin
|
| Label protocol |
According to manufacturer's instructions
|
| |
|
| Hybridization protocol |
According to manufacturer's instructions
|
| Scan protocol |
According to manufacturer's instructions
|
| Description |
Gene expression data from SK-N-SH cell line treated with 100nM of iBET-726
|
| Data processing |
Data was analyzed using Cubic Spline normalisation method
|
| |
|
| Submission date |
May 26, 2013 |
| Last update date |
Aug 23, 2013 |
| Contact name |
Gopinath Ganji |
| E-mail(s) |
gopi@gsk.com
|
| Organization name |
GSK
|
| Street address |
1250 South Collegeville Road
|
| City |
Collegeville |
| State/province |
PA |
| ZIP/Postal code |
19426 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE47386 |
Gene expression study in Neuroblastoma after BET inhibition |
|
