|
| Status |
Public on Aug 26, 2013 |
| Title |
KO618 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse lung Gata5 knockout
|
| Organism |
Mus musculus |
| Characteristics |
tissue: left lung
genotype: Gata5 KO
age: 8-week-old
|
| Treatment protocol |
N/A
|
| Growth protocol |
mouse left lungs were collected from 8-week-old Gata5 KO and wt mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and RNeasy kit cleanup were performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (GeneChip Expression Analysis Technical Manual Rev5, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 35 ug of cRNA were hybridized for 16 hr at 45C in a hybridization oven. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000
|
| Description |
KO biological rep1
|
| Data processing |
Raw data were processed by Affymetrix Expression Console Build v1.3.0.187 using RMA and MAS5 Algorithm. Average intensity data at probe level were background subtracted, log2 transformed, and Quantile normalized.
|
| |
|
| Submission date |
May 28, 2013 |
| Last update date |
Aug 26, 2013 |
| Contact name |
Bohao Chen |
| E-mail(s) |
bchen@medicine.bsd.uchicago.edu
|
| Phone |
773 834 7476
|
| Fax |
773 702 4736
|
| Organization name |
University of Chicago
|
| Department |
Medicine
|
| Street address |
5841 S. Maryland Ave
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE47425 |
Lung transcript expression profile after Gata5 gene deletion |
|
