|
Status |
Public on Aug 26, 2013 |
Title |
WT619 |
Sample type |
RNA |
|
|
Source name |
Mouse lung wild type
|
Organism |
Mus musculus |
Characteristics |
tissue: left lung genotype: Wild Type age: 8-week-old
|
Treatment protocol |
N/A
|
Growth protocol |
mouse left lungs were collected from 8-week-old Gata5 KO and wt mice.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA and RNeasy kit cleanup were performed according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (GeneChip Expression Analysis Technical Manual Rev5, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 35 ug of cRNA were hybridized for 16 hr at 45C in a hybridization oven. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000
|
Description |
WT biological rep1
|
Data processing |
Raw data were processed by Affymetrix Expression Console Build v1.3.0.187 using RMA and MAS5 Algorithm. Average intensity data at probe level were background subtracted, log2 transformed, and Quantile normalized.
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|
|
Submission date |
May 28, 2013 |
Last update date |
Aug 26, 2013 |
Contact name |
Bohao Chen |
E-mail(s) |
bchen@medicine.bsd.uchicago.edu
|
Phone |
773 834 7476
|
Fax |
773 702 4736
|
Organization name |
University of Chicago
|
Department |
Medicine
|
Street address |
5841 S. Maryland Ave
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60637 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE47425 |
Lung transcript expression profile after Gata5 gene deletion |
|