|
| Status |
Public on Jun 01, 2013 |
| Title |
CD31pos-CD45neg-PFV, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
human pluripotent stem cells (hPSCs)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Hematopoietic embryoid bodies after 10 days in hem conditions, CD31 positive CD45 negative
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA using total RNA purification kit (Norgen Biotek, Canada) was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
In accordance with manufacturer's protocols
|
| |
|
| Hybridization protocol |
In accordance with manufacturer's protocols
|
| Scan protocol |
GeneChips were scanned using Affymetrix® Scanner 3000 7G.
|
| Description |
Gene expression data from hematopoietic embryoid bodies after 10 days in hem conditions, sorted on CD31 positive and CD45 negative markers
|
| Data processing |
Data was normalized using Robust Multichip Averaging (RMA) algorithm and baseline transformation to the median of all samples using GeneSpring 6.0 software.
|
| |
|
| Submission date |
May 29, 2013 |
| Last update date |
Jun 01, 2013 |
| Contact name |
Mickie Bhatia |
| E-mail(s) |
mbhatia@mcmaster.ca
|
| Organization name |
McMaster University
|
| Department |
Stem Cell and Cancer Research Institute (SCC-RI)
|
| Street address |
1200 Main Street West
|
| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8N 3Z5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE47466 |
Notch-HES1 signaling axis controls hemato-endothelial fate decisions of human embyronic and induced pluripotent cells |
|
